Serie A champions Juventus saw their 47-match unbeaten home league record come to an end against Udinese.
Defending champions Juventus suffered a shock 1-0 home defeat to Udinese in their Serie A opener on Sunday.
Striker Matej Vydra is back with Watford, this time on a permanent basis after signing a five-year deal to join the club from Udinese.
Juventus have exercised the option to buy Argentine Roberto Pereyra from Udinese, the Serie A champions have announced.
Andrea Stramaccioni will step down as Udinese head coach after just one year when his contract expires at the end of the June.
Cagliari drew a line under 11 years in Serie A by beating Udinese 4-3 in their last game before submitting to relegation.
Fiorentina qualified for the Europa League while Stephan El Shaarawy scored his first goal in seven months for AC Milan.
Liverpool give U21s more game time than Premier League rivals but top flight lags behind Europe.
Sassuolo defeated 10-man Udinese 1-0 on Sunday afternoon to continue their strong finish to the season.
Roma rallied from behind to beat Udinese 2-1 and return to second place in the Serie A table.
Napoli's hopes of Champions League qualification suffered a setback as they drew 2-2 at rock-bottom Parma in Serie A on Sunday.
Roberto Soriano struck twice as Sampdoria halted a six-match winless run with a 4-1 triumph at Udinese.
Udinese ensured they will compete in Serie A again next season after claiming a 1-0 victory at Hellas Verona on Sunday.
Carlos Tevez scored twice as Juventus beat Fiorentina 3-2 as the Serie A leaders closed in on a fourth straight title.
Inter Milan squeezed past nine-man Udinese to climb to seventh place in Serie A with a 2-1 victory at Stadio Friuli.
Udinese delivered a potentially knockout blow to AC Milan's hopes of playing in Europe next season by beating them 2-1.
There was victory for Inter Milan over Roma but AC Milan went down to Udinese in Saturday's Serie A fixtures.
Neither side could find the net in the Milan derby, while Roma missed the chance to go second.
Bostjan Cesar's own goal cost Chievo an important Serie A win at home to Udinese on Sunday as they drew 1-1.
Palermo's long wait for a second Serie A away win of the season came to an end with a 3-1 triumph at Udinese on Sunday.